Talk:Isotopic labeling

	Chemistry portal This article is supported by WikiProject Elements, which gives a central approach to the chemical elements and their isotopes on Wikipedia. Please participate by editing this article, or visit the project page for more details.ElementsWikipedia:WikiProject ElementsTemplate:WikiProject Elementschemical elements articles
Mid	This article has been rated as Mid-importance on the importance scale.
	This article is supported by the Isotope Taskforce.

Spectroscopy (inactive)

This article is within the scope of WikiProject Spectroscopy, a project which is currently considered to be inactive.SpectroscopyWikipedia:WikiProject SpectroscopyTemplate:WikiProject SpectroscopySpectroscopy articles

Chemistry Mid‑importance

	This article is within the scope of WikiProject Chemistry, a collaborative effort to improve the coverage of chemistry on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.ChemistryWikipedia:WikiProject ChemistryTemplate:WikiProject ChemistryChemistry articles
Mid	This article has been rated as Mid-importance on the project's importance scale.

Mass spectrometry (inactive)

This article is within the scope of WikiProject Mass spectrometry, a project which is currently considered to be inactive.Mass spectrometryWikipedia:WikiProject Mass spectrometryTemplate:WikiProject Mass spectrometryMass spectrometry articles

Comments from Synthetic Circuits (M.Kang) for ENCH468M[edit]

Summary The article summarizes the use of stable isotope labeling as a method for flux quantification. It introduces a model network and diagrams the passage of mixtures of carbon isotopes (specifically glucose conversion) through the pentose phosphate pathway.

Major Comments: The article needs a more complete introduction of carbon isotopes and their chemical background, possibly in the form of internal links to other Wikipedia pages and a short exposition on the background of metabolic fluxes. The rest of the article talks about isotopes in some degree, but the article itself concerns itself only with biological functions – it needs to be integrated better. The claims made in the article also need sources – these are easy in Wikipedia by simply writing ^[1] in the page to automatically format endnotes.

Minor Comments: Some cleanup needs to be done, such as properly captioning the images, and capitalizing words unnecessarily, but the content in general seems to be complete. Tagelied (talk) 22:29, 30 November 2011 (UTC)[reply]

References

^ Voight, C.A. “Edge Detetction in…” Cell 12, 29 May 2000

Comments form group W[edit]

Majors problems: The thing that stuck out was the captions for figures 1 and 2. The figures were good but captions were out of order and did not indicate that the circles represented certain carbon atoms. Words such as NMR, MS, Proton NMR, isotopomer and GC-MS should be briefly explained. It is better to be careful about using abbreviations. “Proton NMR was the first technique that was used for 13C-labeling experiments.” Citations are needed especially the testing isotopes part.

Some points that might help:

You could reduce the description of the various ways to measure isotope labeling, such as radioisotopes labelling and GC-MS etc. You should mention them, but just shorten their description. This article needs a clear explanation of stable isotope labeling and how isotopes are incorporated. The terms such as F6P,T3P,G6P, 2,3-13C T3P should be expanded for someone who is not in this class. It has also some parts that should be put together (Metabolic flux analysis, isotope tracer).“Metabolic pathway analysis … to figuring out the metabolic pathways and reactions that occur within a cell.” Instead, figuring out metabolic pathways that occur within a cell makes sense. In the 'metabolic pathways analysis' part, explain how successful stable isotope labeling is used to elucidate metabolic pathways by giving examples from published papers. You might want to focus more on isotopomer balances (the class note and handout might be helpful), applications to metabolic engineering, possible examples of successful isotope labeling in metabolic engineering. You should be careful about terms that you introduce without giving inadequate explanation. I am not sure if proteomics is a branch of genetics. The 'other use of metabolic engineering' part needs some work.There are some wiki articles that explain some terms in that part. — Preceding unsigned comment added by 71.163.114.136 (talk) 06:48, 1 December 2011 (UTC)[reply]

Instructor review

In general, you need to cite more references and at more places than what you have done.

Your article needs hyperlinks to other Wikipedia articles. I am sure there are Wikipedia articles on NMR, MS, etc. that you can link to.

The title "Testing the isotopes" is a little vague. Be more specific.

The sentence beginning with "13C NMR spectrum allows a more detailed isotopomer distribution" is hyperspecialized and hard to follow without a figure. Don't draw a figure, but condense it and make it more lucid so that the message comes across.

There are myriad methods to derivatize compounds for MS. You should mention the ones you list as examples.

Figure numbers are not used in Wikipedia (as far as I know). See Wikipedia featured articles to get an example.

Don't italicize and capitalize when not necessary. For example the expansions of acronyms do not have to be capitalized: write "nuclear magnetic resonance (NMR)" and not "Nuclear Magnetic Resonance (NMR)".

Don't use jargon: "carbon atom", not "carbon"

Don't write directly to the reader, as in "let us assume ..."

The expressions "meaning that" and "last but not least" seems a little awkward. Reword.

UM BIOE120 Instructor (talk) 05:27, 14 December 2011 (UTC)[reply]

External links modified[edit]

Hello fellow Wikipedians,

I have just added archive links to 2 external links on Isotopic labeling. Please take a moment to review my edit. If necessary, add {{cbignore}} after the link to keep me from modifying it. Alternatively, you can add {{nobots|deny=InternetArchiveBot}} to keep me off the page altogether. I made the following changes:

Added archive https://web.archive.org/20120415075154/http://hort.ifas.ufl.edu/teach/guyweb/bot6516/PentosePhosphatePathway.pdf to http://hort.ifas.ufl.edu/teach/guyweb/bot6516/PentosePhosphatePathway.pdf
Added archive https://web.archive.org/20120404143011/http://www.perkinelmer.com/Catalog/Category/ID/Stable%20Isotope%20Labeling to http://www.perkinelmer.com/Catalog/Category/ID/Stable%20Isotope%20Labeling

When you have finished reviewing my changes, please set the checked parameter below to true to let others know.

This message was posted before February 2018. After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than regular verification using the archive tool instructions below. Editors have permission to delete these "External links modified" talk page sections if they want to de-clutter talk pages, but see the RfC before doing mass systematic removals. This message is updated dynamically through the template {{source check}} (last update: 18 January 2022).

If you have discovered URLs which were erroneously considered dead by the bot, you can report them with this tool.
If you found an error with any archives or the URLs themselves, you can fix them with this tool.

Cheers.—^{cyberbot II}_{Talk to my owner:Online} 01:18, 13 February 2016 (UTC)[reply]

Problem with placement of references Yesterday I added a new sub-section ("Applications in human mineral nutrition research") to the "Stable isotope labeling" section and for some reason the references were placed at the end of this section instead of at the end of the full article. I assume this is an error but I am unsure of how to correct it. Thanks. Lvaughn7 (talk) 18:40, 30 May 2020 (UTC)[reply]

[1] Voight, C.A. “Edge Detetction in…” Cell 12, 29 May 2000

[1]