User:Yates9/molecular biology techniques
Basic Molecular Biology Techniques
[edit]Learning Neuroscience
[edit]- Creating a Wikibook Notes
- Books for experts
- Imaging Technology
- Quantum dot semiconductor nano-crystal that is sensitive to light, usually UV. Less susceptible to photobleaching, can measure Ph surrounding it.
- FRET imaging Förster resonance energy transfer: technique to visualize interactions between especially marked molecules, which emit florescence as part of interaction
- Entries following need serious review.. many misclassified etc..
- Arabidopsis and Botany Protocols
- Protoplast Isolation Protocol unfound
- DNA
- Molecular cloning (DNA Cloning) isolating a defined DNA sequence and obtaining multiple copies of it in vitro
- Gel Electrophoresis (DNA also Agarose)technique used for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein molecules using an electric field applied to a gel matrix
- DNA Thermodynamics, Hybridization
- Membrane Hybridization Screening of Libraries ?
- DNA sequencing and Maxam-Gilbert Sequencing
- Molecular cloning or Gene Cloning
- Restriction Enzyme (also Digestion)
- DNA Transformation - Contains History of DNA
- Electrophoretic Mobility Shift Assay
- General
- Gel Electrophoresis
- Liquid Scintillation Counting work with Beta particles emitted from the sample transfer energy to the solvent molecules, which in turn transfer their energy to the fluors; the excited fluor molecules dissipate the energy by emitting light
- Autoradiograph with Phosphorimaging just reference
- Non-radioactive detection methods and tracers.
- HPLC - High Performance Liquid Chromotography
- Immunoprecipitation
- Immunoprecipitation Background
- Immunoprecipitation Protocol
- Mass Spectrometry
- Mass Spectrometery
- Edman Degradation
- Electrospray MS
- Microarray
- Protein Microarray Theory
- PCR - Polymerase Chain Reaction
- Theory and Development of PCR
- Avoiding PCR Contamination
- PCR Primer Design Guidelines and Software
Protein Techniques
- Protein Identification
- Protein Purification
- Protein Separation
- Western Blot
- Immunoprecipitation IP
- Proteomics Methods
- Recombinant Protein Expression
- Recombinant Protein Expression
- RNAi and SiRNA
- SiRNA Transfection Protocol
- SDS-PAGE Gel Electrophoresis
- SDS-PAGE Gel Electrophoresis Background Theory
- Trypsin Digestion (In Gel Trypsin Digestion)
- In Gel Trypsin Digestion Theory
- Transgenic Plants and Animals
- Transgenic Animals
- X-Ray Crystallography
- X-ray Crystallography
- Gene (DNA) Sequencing
- Polymerase chain reaction
- contigs
- Gene Expression
- Probes, Hybridization
- Microarray