User:Shashgundi/Armando J. Parodi

Armando J. Parodi was born on March 16, 1942 in Buenos Aires, Argentina, and is a prominent name in the field of glycobiology. He did his initial education at the School of Sciences of the University of Buenos Aires. He did his PhD work under Luis Federico Leloir, a recipient of the Nobel Prize in Chemistry for his work involving the finding of sugar nucleotides and how they play a role in the making of oligosaccharides and polysaccharides. He also pursued postdoc work at the Pasteur Institute in Paris, France and Duke University in Durham, NC, USA.

Early life and education

Although his father was a physician who worked more in academia than in a clinic, the lack of strong science teachers in secondary school led him to become more interested in politics. However, when he graduated high school, Parodi attended the School of Sciences at the University of Buenos Aires. The reason for this, was his interest in Chemistry that developed as a result of having a single passionate Chemistry teacher in secondary school. Due to the restructuring of the Higher Education system as a result of Juan Peron’s dictatorship, many of his professors were younger and recent graduates of post-doctoral programs in the US and Europe. During his final year at the University of Buenos Aires, his father suggested that he should pursue his PhD at the Fundacion Instituto Leloir, and so he enrolled in the Biochemistry course there. While working under Luis. F. Leloir, Parodi saw that Leloir was able to synthesize particulate glycogen de novo and that influenced the direction of his future research.

Parodi also completed a 2 year postdoctoral fellowship at the Pasteur Institute in Paris, France.

Research and career

While working under Luis F. Leloir, Parodi’s research was involved with the synthesis of particulate glycogen de novo using UDP-Glc and rat livers for glycogen synthase.

In 1970, he joined Leloir and Nicolas Behrens in their research that was involved with the incubation of dolichol-P-Glc using liver microsomes to transfer glucose to a dolichol-P-P-linked glycan known as Glc3Man9GlcNAc2-P-P-dolichol.

After completing his fellowship in Paris, and returning to Buenos Aires, between the years of 1975 and 1978, he conducted research involved with demonstrating the presence of a dolichol-P-dependent pathway of N-glycosylation in yeast. However, a paper in 1980 that stated the lack of free or sugar-bound dolichol-P in trypanosomatid protozoa meant that the pathway he discovered was not in the organism. This lead him to do his own research with trypanosomatids and using ¹⁴C Glucose he found that the synthesis of dolichol-P-P-glycans in protozoa was possible but glucose was lacking in the formed glycans.

Further research into glycans in trypanosomes, specifically Trypanosoma cruzi, was conducted by Parodi. Using short pulses of ¹⁴C, three protein glycans were produced and they were known as Glc₁Man₉GlcNAc₂, Glc₁Man₈GlcNAc₂, and Glc₁Man₇GlcNAc₂. These were then replaced by Man₉GlcNAc₂, Man₈GlcNAc₂, Man₇GlcNAc₂, and Man₆GlcNAc₂ in the mature versions of the glycoproteins. This led to the conclusion that the transfer of a glucose to the Man₉GlcNAc₂ was the only way that Glc₁Man₉GlcNAc₂ could have been made.

Parodi also looked into how glucosylation/deglucosylation in the ER’s lumen were involved with the correct folding of N-linked glycoproteins. His research led him to find that misfolding allowed for conformations that would allow for glycoproteins to act as glucose acceptors. He was able to find this using UDP-Glc: glycoprotein GT that worked as ways of sensing the various conformations of the glycoproteins. The sensitive of GT was very helpful as it was able to detect formations that were undetectable via other methods. This work was crucial in discovering how protein folding was controlled. By looking at how GT encouraged proper folding, the enzyme was found as a stress protein and there was increased synthesis of it during periods of stress for the ER.

Recent work by Parodi has looked at how Glucosidase I removes glucose from Glc3Man9GlcNAc2. He has looked at how glycosylation MOGS-CDG leads to Glucosidase 1 encoding gene mutations. Absence of this GI has been linked to death in certain yeasts, specifically, Schizosaccharomyces pombe. Parodi and his team wanted to look at this mutation and understand what was causing the defects in these Δgls1-S cells.

Awards and honors

• “Bernardo Houssay” Award in Biology (National Council of Scientific and Technical Research, Argentina, 1987)
• Award in Biology (Third World Academy of Sciences, Trieste, Italy, 1994)
• International Research Scholar, Howard Hughes Medical Institute (1997-2011)
• Award Venancio Deulofeu in Chemistry and Biochemistry of Carbohydrates, National Academy of Exact, Physical and Natural Sciences (1998)
• Honorary Member, Spanish Society of Biochemistry and Molecular Biology (2001)
• Konex Platinum Award in Science and Technology-Biochemistry and Microbiology (1993-2002). Konex Foundation, Buenos Aires (2003)
• Bunge y Born Foundation Award in Biological Chemistry (2005)
• Karl Meyer Award in Glycobiology (2011)

References

Category:1926 births Category:Nobel laureates in Chemistry Category:American Nobel laureates Category:Jewish Nobel laureates Category:Nobel laureates affiliated with Missouri Category:Abraham Lincoln High School (Brooklyn) alumni Category:American biochemists Category:Jewish biochemists Category:Fellows of the American Academy of Arts and Sciences Category:Foreign Members of the Royal Society Category:HIV/AIDS researchers Category:Jewish American scientists Category:Jewish chemists Category:Living people Category:Members of the European Molecular Biology Organization Category:Members of the French Academy of Sciences Category:Members of the United States National Academy of Sciences Category:National Medal of Science laureates Category:Members of the Pontifical Academy of Sciences Category:Pennsylvania State University alumni Category:Scientists from Brooklyn Category:Stanford University School of Medicine faculty Category:Institute for Advanced Study visiting scholars Category:Fellows of Clare Hall, Cambridge Category:Alumni of Clare Hall, Cambridge Category:Biotechnologists Category:Recipients of the Albert Lasker Award for Basic Medical Research Category:Scientists from New York (state)

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