User:Kaitlynwu18/Termination signal/Alib2022 Peer Review

Peer review

Complete your peer review exercise below, providing as much constructive criticism as possible. The more detailed suggestions you provide, the more useful it will be to your classmate. Make sure you consider each of the following aspects:

Lead

Guiding questions:

Has the Lead been updated to reflect the new content added by your peer?
Does the Lead include an introductory sentence that concisely and clearly describes the article's topic?
Does the Lead include a brief description of the article's major sections?
Does the Lead include information that is not present in the article?
Is the Lead concise or is it overly detailed?

Content

Guiding questions:

Is the content added relevant to the topic?
Is the content added up-to-date?
Is there content that is missing or content that does not belong?
Does the article deal with one of Wikipedia's equity gaps? Does it address topics related to historically underrepresented populations or topics?

Tone and Balance

Guiding questions:

Is the content added neutral?
Are there any claims that appear heavily biased toward a particular position?
Are there viewpoints that are overrepresented, or underrepresented?
Does the content added attempt to persuade the reader in favor of one position or away from another?

Sources and References

Guiding questions:

Is all new content backed up by a reliable secondary source of information?
Does the content accurately reflect what the cited sources say? (You'll need to refer to the sources to check this.)
Are the sources thorough - i.e. Do they reflect the available literature on the topic?
Are the sources current?
Are the sources written by a diverse spectrum of authors? Do they include historically marginalized individuals where possible?
Are there better sources available, such as peer-reviewed articles in place of news coverage or random websites? (You may need to do some digging to answer this.)
Check a few links. Do they work?

Organization

Guiding questions:

Is the content added well-written - i.e. Is it concise, clear, and easy to read?
Does the content added have any grammatical or spelling errors?
Is the content added well-organized - i.e. broken down into sections that reflect the major points of the topic?

Images and Media

Guiding questions: If your peer added images or media

Does the article include images that enhance understanding of the topic?
Are images well-captioned?
Do all images adhere to Wikipedia's copyright regulations?
Are the images laid out in a visually appealing way?

For New Articles Only

If the draft you're reviewing is for a new article, consider the following in addition to the above.

Does the article meet Wikipedia's Notability requirements - i.e. Is the article supported by 2-3 reliable secondary sources independent of the subject?
How exhaustive is the list of sources? Does it accurately represent all available literature on the subject?
Does the article follow the patterns of other similar articles - i.e. contain any necessary infoboxes, section headings, and any other features contained within similar articles?
Does the article link to other articles so it is more discoverable?

Overall impressions

Guiding questions:

Has the content added improved the overall quality of the article - i.e. Is the article more complete?
What are the strengths of the content added?
How can the content added be improved?

Examples of good feedback

A good article evaluation can take a number of forms. The most essential things are to clearly identify the biggest shortcomings, and provide specific guidance on how the article can be improved.

Peer review of "Homemaking"
Peer review of this article about a famous painting

Additional Resources

Check out the Editing Wikipedia PDF for general editing tips and suggestions.

General info[edit]

Whose work are you reviewing?

Kaitlynwu18

Link to draft you're reviewing: https://en.wikipedia.org/wiki/User:Kaitlynwu18/Termination_signal?veaction=edit&preload=Template%3ADashboard.wikiedu.org_draft_template
Link to the current version of the article (if it exists): Termination signal

Evaluate the drafted changes[edit]

(Compose a detailed peer review here, considering each of the key aspects listed above if it is relevant. Consider the guiding questions, and check out the examples of what feedback looks like.)

Lead

"A termination signal is a sequence that signals the end of transcription or translation." Good and clear definition.

"It is needed because only gene-encoding parts of the chromosome should be transcribed. Termination signals bring a stop to transcription, ensuring that genes are properly transcribed." This seems a little repetitive, maybe find a way to merge the two sentences?

"Transcription begins at the promoter when RNA polymerase, an enzyme that facilitates transcription of DNA into mRNA, binds to a promoter, unwinds the helical structure of the DNA, and uses the single-stranded DNA as a template to synthesize RNA." This sentence is a little confusing to read for me, maybe arrange it a little differently (like making it two sentences or use the commas differently). Or maybe define DNA polymerase before the sentence so that it does not take away from what you are trying to say about transcription.

For the lead section, maybe add a brief description of the other sections (this is based on the peer review prompt).

Polymerase Read-Through

"Termination signals cause the release of RNA polymerase from the growing RNA transcript, ensuring that transcription only occurs for parts of the DNA sequence that are meant to be expressed by the cell.^[1] Termination signals are important aspects of gene regulation because they mark the end of a gene transcript.^[1] Thus, inhibition or activation of termination can change expression levels of certain genes." Maybe ask our TA for advice on this but I don't know if you have to cite the article after each sentence if the whole paragraph uses the same reference.

"Antitermination involves the inhibition of terminators, thus preventing RNA polymerase from detaching from the RNA in response to a termination signal and increasing downstream gene expression" Maybe can improve wording here? The part I bolded seems a little out of place?

"Two types of termination signals in bacteria are intrinsic and factor-dependent terminators.^[2] Intrinsic termination occurs when a specific sequence on the RNA elicits detachment of RNA polymerase from the RNA-DNA complex.^[2] In E. coli, one termination signal consists of an RNA hairpin that has high amounts of guanine and cytosine as well as a region high in uracil nucleobases.^[2] Factor-dependent terminators require proteins for proper termination.^[2] Rho-dependent termination is a common termination mechanism found in bacteria that involves the binding of Rho protein to remove RNA polymerase from the DNA-RNA complex.^[2]" I like the definition and examples for the two types of termination signals.

I like the information on antitermination and how it can be important.

Termination Signal Efficiency

"Termination efficiency of T7 RNA polymerase is around 74%, which creates issues when T7 RNA polymerase is used to produce recombinant proteins." What is the T7 RNA and why is it important to discuss? (ie why did you choose this one to talk about?) Is there more general information about this?

"Selectable marker genes that are downstream of the target gene insertion site and genes that encode regulatory proteins may have altered expression as a result.^[3] Hence, proper termination of transcription is needed for plasmid stability in host cells for the proper production of recombinant proteins." Good explanation of the importance of proper termination of transcription.

Translational Termination Signals

No content here? Did you forget to add this or remove the section?

Overall, I think it is a good introduction into termination signals. I also liked the pictures that you used. Most of what I caught were just areas where the wording or grammar can be improved. If you are looking for more ideas on content that you can add, maybe have a section on application or why we care about being able to understand it. I think that your additions will definitely add to the current article. Nice job!

^ ^a ^b Santangelo, Thomas; Artsimovitch, Irina (9 May 2011). "Termination and antitermination: RNA polymerase runs a stop sign". Nature Reviews Microbiology. 9: 319–329 – via PubMed.
^ ^a ^b ^c ^d ^e Santangelo, Thomas; Artsimovitch, Irina (9 May 2011). "Termination and antitermination: RNA polymerase runs a stop sign". Nature Reviews Microbiology. 9: 319–329 – via PubMed.
^ Mairhofer, Juergen; Wittwer, Alexander; Cserjan-Puschmann, Monika; Striedner, Gerald (2015-03-20). "Preventing T7 RNA Polymerase Read-through Transcription—A Synthetic Termination Signal Capable of Improving Bioprocess Stability". ACS Synthetic Biology. 4 (3): 265–273. doi:10.1021/sb5000115.

[:02-1] Santangelo, Thomas; Artsimovitch, Irina (9 May 2011). "Termination and antitermination: RNA polymerase runs a stop sign". Nature Reviews Microbiology. 9: 319–329 – via PubMed.

[:022-2] Santangelo, Thomas; Artsimovitch, Irina (9 May 2011). "Termination and antitermination: RNA polymerase runs a stop sign". Nature Reviews Microbiology. 9: 319–329 – via PubMed.

[:1-3] Mairhofer, Juergen; Wittwer, Alexander; Cserjan-Puschmann, Monika; Striedner, Gerald (2015-03-20). "Preventing T7 RNA Polymerase Read-through Transcription—A Synthetic Termination Signal Capable of Improving Bioprocess Stability". ACS Synthetic Biology. 4 (3): 265–273. doi:10.1021/sb5000115.

[1]

[2]

[3]