User:Januoaxe/sandbox
| Januoaxe/sandbox | |
|---|---|
| Scientific classification | |
| Domain: | |
| Kingdom: | |
| Phylum: | |
| Class: | |
| Order: | |
| Family: | |
| Genus: | |
| Species: | S. acidocaldarius
|
| Binomial name | |
| Sulfolobus acidocaldarius Brock et al. 1972
| |
Sulfolobus acidocaldarius is an Archaea belonging to the kingdom Crenarchaeota. S. acidocaldarius was the first species belonging to the genus Sulfolobus to be described, in 1972 by Thomas Brock and colaborators[1]. This species was found to grow optimally between 75 and 80ºC, with pH optimum in the range of 2-3.
Isolation[edit]
S. acidocaldarius was first isolated from thermal soils and hot springs with low pH in the United States of America (specifically in the Yellowstone National Park), in El Salvador, Dominica and Italy. The springs where this species was isolated had a pH less than 3 and temperatures in the range of 65-90ºC[2].
Morphological Description[edit]
S. acidocaldarius is, as all Archaea, unicellular. Cells belonging to this species are spherical, albeit irregular, and usually possess lobes. The diameter of the cells fall in the range of 0.8-1 μm, with little size variation[3].
Metabolism[edit]
S. acidocaldarius is a facultative autotroph. When growing autotrophically this organism oxidises sulfur to sulfate, while fixating carbon from carbon dioxide. The doubling time of cultures growing on sulfur alone falls between 36.8-55.3h. This species can also grow on complex organic substrates. When growing on 0.1% yeast extract the growth is faster, and the doubling times are between 6.5 to 8h[4][5].
Genome[edit]
In 2005 the complete genome of Sulfolobus acidocaldarius strain DSM639 was published[6]. The genome of this crenarchaeon is composed of a single circular chromosome with 2,225,959-bp, with a G+C content of 36.7%. The authors predicted 2292 protein-coding genes. The genome of Sulfolobus acidocaldarius is very stable, with little, if any, rearrangements due to mobile elements.
The authors found the genes necessary for the synthesis of purines and pyrimidines, as well as for all amino acids except for selenocysteine. Genes for glucose metabolism suggest the existence of two alternative pathways. This Sulfolobus species grows on a more limited range of carbon sources, relative to other Sulfolobus species, and this might be due to the lack of adequate transporters.
Significance[edit]
The restriction enzyme SuaI is obtained from this organism[7].
References[edit]
- ^ Brock, TD; Brock, KM; Belly, RT; Weiss, RL (1972). "Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature". Archiv fur Mikrobiologie. 84 (1): 54–68. PMID 4559703.
- ^ Brock, TD; Brock, KM; Belly, RT; Weiss, RL (1972). "Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature". Archiv fur Mikrobiologie. 84 (1): 54–68. PMID 4559703.
- ^ Brock, TD; Brock, KM; Belly, RT; Weiss, RL (1972). "Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature". Archiv fur Mikrobiologie. 84 (1): 54–68. PMID 4559703.
- ^ Brock, TD; Brock, KM; Belly, RT; Weiss, RL (1972). "Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature". Archiv fur Mikrobiologie. 84 (1): 54–68. PMID 4559703.
- ^ Shivvers, DW; Brock, TD (May 1973). "Oxidation of elemental sulfur by Sulfolobus acidocaldarius". Journal of bacteriology. 114 (2): 706–10. PMID 4706192.
- ^ Chen, L; Brügger, K; Skovgaard, M; Redder, P; She, Q; Torarinsson, E; Greve, B; Awayez, M; Zibat, A; Klenk, HP; Garrett, RA (July 2005). "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota". Journal of bacteriology. 187 (14): 4992–9. PMID 15995215.
- ^ Prangishvili, DA; Vashakidze, RP; Chelidze, MG; Gabriadze, IYu (11 November 1985). "A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius". FEBS letters. 192 (1): 57–60. PMID 2996942.