User:Abrar muhammed/Books/PGE 2

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UNIT - I SCOPE OF GENETIC ENGINEERING: Genetic engineering; DNA; Gene; RNA; Transcription (genetics); Medical genetics; Gene therapy; Human Genome Project; Genetically modified crops; Recombinant DNA; Molecular cloning; Restriction map; Polymerase chain reaction; Nucleic acid test; Applications of PCR; Real-time polymerase chain reaction
UNIT - II GENE CLONING STRATEGIES: Restriction enzyme; Polymerase; Reverse transcriptase; Ligase; Alkaline phosphatase; Nuclease; Cloning vector; Aspergillus nuclease S1; Plasmid; Bacteriophage; Cosmid; Bacterial artificial chromosome; Phagemid; Artificial chromosome; Yeast artificial chromosome; Human artificial chromosome; Base pair; Transformation (genetics); Library (biology); CDNA library; Genomic library; Linker DNA; Site-directed mutagenesis; Mutagenesis (molecular biology technique); Protein engineering
UNIT - III GENE EXPRESSION: Gene expression; Hybrid (biology); Northern blot; Nuclease protection assay; Reporter gene; Assay; Primer extension; DNA microarray; Microarray; Protein microarray; Phage display; Two-hybrid screening
UNIT - IV TRANSGENIC TECHNOLOGY: Transgenesis; Transposon tagging; Gene knockout; Gene targeting; Chromosome engineering; Gene silencing
UNIT - V GENONIMCS AND FORENSICS: Nucleic acid sequence; Forensic science; Enzyme; Genetic disorder; DNA profiling